How to Reconstitute HCG Powder: Step-by-Step Guide

Human Chorionic Gonadotropin (HCG) is a hormone commonly used for fertility, hormone support, and certain weight management protocols. Most HCG products come in lyophilized powder form that must be reconstituted with bacteriostatic water or saline before injection.

Proper reconstitution is essential to ensure the correct dosage, potency, and safety. This guide explains the process in easy-to-follow steps for both beginners and experienced users.

What You Need for Reconstitution

Before starting, prepare the following items:

• Lyophilized HCG powder vial
• Bacteriostatic water (or sterile saline as directed)
• Alcohol swabs
• Insulin syringes (typically 1 mL)
• Clean, flat workspace

⚠️ Always follow your healthcare provider’s instructions for dosing and preparation.

Step-by-Step Reconstitution Guide

Step 1: Wash Your Hands

• Clean hands thoroughly with soap and water.
• Use an alcohol swab on your workspace to maintain sterility.

Step 2: Clean the Vials

• Wipe the rubber stopper of the HCG powder vial and the bacteriostatic water vial with an alcohol swab.

Step 3: Draw Bacteriostatic Water

• Use an insulin syringe to draw the prescribed amount of bacteriostatic water.
• Common ratios: 1 mL, 2 mL, or 3 mL depending on the desired concentration.

Step 4: Add Water to HCG Powder

• Slowly inject the bacteriostatic water into the HCG vial along the side to avoid foaming.
• Do not shake vigorously — gently swirl the vial until powder dissolves completely.

Step 5: Check the Solution

• The reconstituted HCG should be clear and free of particles.
• Discard if cloudy or contains visible particles.

Step 6: Store Properly

• Keep the reconstituted vial refrigerated at 2–8°C (36–46°F).
• Typically stable for 30–60 days depending on manufacturer instructions.

Calculating Dosage After Reconstitution

Once reconstituted, the concentration of HCG is determined by the ratio of powder to water:

• Example: 10,000 IU vial + 1 mL water = 10,000 IU/mL
• For a 250 IU dose, draw 0.025 mL with an insulin syringe

Always double-check your calculations before injection.

Injection Guidelines

• Subcutaneous injection is most common for HCG.
• Alternate injection sites (abdomen, thigh) for comfort.
• Follow sterile technique to avoid infection.

Tips for Safe Handling

• Use a new syringe for each injection.
• Avoid touching the needle or vial stopper with your hands.
• Discard vials past their expiration or if stored improperly.
• Monitor for any signs of contamination (cloudiness, particles).